File Info : tien

Contents : C. Preparation of Columns 30.1.12 AOAC Official Method 979.11 Caffeine in Roasted Coffee Chromatographic Spectrophotometric Method First Action 1979 (Applicable to regular and decaffeinated coffee.) A. Reagents and Apparatus (a) Caffeine standard solutions. 10 20 and 30 mg caffeine/mL. Accurately weigh 100 mg caffeine (USP anhydrous) into 100 mL volumetric flask dissolve in CHCl3 and dilute to volume with CHCl3. Dilute 10 mL aliquot of this solution to 100 mL with CHCl3. Further dilute 10 20 and...[more]

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